Failed Autoclave Tests - What to do?

Don't Panic! There only a few ways autoclave testing can FAIL and below is the types of failure, causes and next steps.

Failed Biological/Spore Test

spore-test

Biological tests are usually considered the most important test as they best reflect whether biological materials are being.

Operator error is the most common reason for a failed spore test. If your autoclave has automatic controls and completing cycles without giving any failure messages, the chances are that the causes of a failed spore test due to some type of operator error. You may need to review the sterilization procedures your staff has been using to see if you could identify the problem. Firstly you have to make sure that they are not overloading the Autoclave. Check the manufacturer’s specifications in regards to how the sterilizer should be loaded. The next thing you will need to check is the time and temperature settings. It is possible that the factory default settings somehow got changed or the operator is simply not running the sterilizer hot enough to long enough. Manufactures default settings can vary a little but generally an autoclave has to maintain 270F degrees Fahrenheit for 12 minutes. Lastly if the Autoclave is a manual unit with manual controls, you will need to monitor and record"

Common-Causes-of-Ster--1-

Operator error is the most common reason for a failed spore test. If your autoclave has automatic controls and completing cycles without giving any failure messages, the chances are that the causes of a failed spore test due to some type of operator error. You may need to review the sterilization procedures your staff has been using to see if you could identify the problem. Firstly you have to make sure that they are not overloading the Autoclave. Check the manufacturer’s specifications in regards to how the sterilizer should be loaded. The next thing you will need to check is the time and temperature settings. It is possible that the factory default settings somehow got changed or the operator is simply not running the sterilizer hot enough to long enough. Manufactures default settings can vary a little but generally an autoclave has to maintain 270F degrees Fahrenheit for 12 minutes. Lastly if the Autoclave is a manual unit with manual controls, you will need to monitor and record

Failed Control Test

To ensure that the micro organisms are working it is required that a positive control tube or vial is set aside (a bacterial vial that hasn't been in the autoclaved). If the control vial does not go through a color change, that means that the control vial was not viable to begin with.

Reasons that it could fail is:

  • Enter
  • Enter?

Failed Chemical Test

Check the mode? Check the manufacturer settings?